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ZenBio
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Thermo Fisher
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Jingmei Biotech Co Ltd
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ZenBio
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Bioss
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STEMCELL Technologies Inc
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Journal: International Journal of Fertility & Sterility
Article Title: Extracellular Vesicles Present in Bone Marrow Mesenchymal Stromal/Stem Cell Conditioned Media Restore Spermatogenesis in Azoospermic Mice
doi: 10.22074/IJFS.2024.2021445.1619
Figure Lengend Snippet: Validation of MSCs and EVs derived from BMCM isolations was performed. The adherence and morphology of the cells were assessed, with A. BMMSCs and B. AT-MSCs. C. Differentiation into adipogenic lineages was observed for BM-MSCs and D. AT-MSCs, E. While osteogenic differentiation was confirmed for BM-MSCs and F. AT-MSCs. G. Chondrogenic differentiation was also achieved, as shown for BM-MSCs and H. AT-MSCs. I, J. Flow cytometry analysis revealed that both BM-MSCs and AT-MSCs expressed surface markers CD44 and CD105 at levels exceeding 95%, while hematopoietic markers CD34 and CD45 were expressed at levels below 10%. K. The isolation of EVs was confirmed using SEM and TEM, along with a detailed analysis of EV diameter distribution (scale bar A-H: 500 µm). MSCs; Mesenchymal stromal/stem cells, EVs; extracellular vesicles, BMCM; Bone marrow-derived mesenchymal stomal/stem cells conditioned medium, AT; Adipose tissue, SEM; Scanning electron microscope, and TEM; Transmission electron microscope.
Article Snippet: This procedure involved incubating 5×10 5 cells from the third passage with monoclonal antibodies specific to surface markers CD34, CD45, CD44, and
Techniques: Biomarker Discovery, Derivative Assay, Flow Cytometry, Isolation, Microscopy, Transmission Assay